Ear endoscope applied in otosclerosis artiifcial stapes surgery / 中国内镜杂志

Objective To investigate the application of ear endoscopic in artificial stapes surgery of otosclerosis.Methods Artificial stapes surgery was performed by two amplification device, microscope or ear endoscope, in 64 cases of patients with otosclerosi. Then compare the operation time and analyze the effect of surgery.Results Patients postoperative hearing of the two groups were signiifcantly improved, the difference of air bone conduction was statistically signiifcant (P 0.05).Conclusion Compared with microscopic surgery, ear endoscope artificial stapes surgery has its’ unique advantages with clear image and small trauma.

Inhibitory effects of sulforaphane on hypoxia-inducible factor-1α in human prostate cancer cells / 中华泌尿外科杂志

Objective To investigate the effects of sulforaphane (SFN) on the proliferation of prostate cancer DU145 cells and hypoxia-inducible factor-1α(HIF-lα) expression,and to explore the possibility of SFN in the prevention of prostate cancers.Methods The DU145 cells were treated with different doses and different time of SFN.The cell proliferation viability were measured by 3-(4,5)-dimethylthiahiazo (-zy1)-3,3,5-di-phenytetrazo`liumromide (MTT) proliferation and cytotoxicity assay kit.The hypoxia tumor microenvironment was stimulated by pretreating cells with 1％ O2,HIF-lα expression and vascular endothelial growth factor (VEGF) activitity were detected by Western-blot and ELISA,respectively.Results SFN had an inhibitory effect on the proliferation of human prostate cancer DU145 cells in a dose-and time-dependent manner.The cell proliferation inhibition rate exceeded 50％ in more than 20 μ mol/L SFN-treated groups after 48 hours.SFN could inhibit the expression of HIF-1α induced by 1％ O2 and VEGF activity.10 μmol/L SFN showed inhibitory effect on the expression of HIF-1α after 24 hours.20 μ mol/L SFN showed a significant inhibitory effect on the expression of HIF-1α after 12 hours.And peroxidase had synergistic inhibition on HIF-1α.Conclusions SFN can inhibit the proliferation of DU145 prostate cancer cells and HIF-lα expression.SFN can be used as a potential chemotherapeutic agent against human prostate cancer.